GRP94 antibody (C-Term)

Catalog No. ABIN1686653

This Rabbit Polyclonal antibody specifically detects GRP94 in WB, IHC, IF, IP and ICC. It exhibits reactivity toward Mouse.

Quick Overview for GRP94 antibody (C-Term) (ABIN1686653)

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRP94 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-GRP94 Antibody

Binding Specificity: C-Term

Specificity: Detects ~94 kDa.

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Peptide Affinity Purified

Immunogen: Synthetic peptide corresponding to the sequence near the C-terminus of mouse GRP94

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:120)
• IP (1:80)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN1686652 was sufficient for detection of Grp94 in 20 μg of Hela lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Alternative Name: GRP94

Background: Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2). Grp94 is a member of the HSP90 family of stress proteins and shares sequence homology with its cytosolic equivalent, HSP90 (3). Both HSP90 and Grp94 are calcium binding proteins (4). Despite sharing 50 % sequence homology over its N domains and complete conservation in its ligand binding domains with HSP90, Grp94 and HSP90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolyisis activity (5). Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).

Gene ID: 22027

NCBI Accession: NP_035761

UniProt: P08113

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades