GATA3 antibody (pSer162) (AbBy Fluor® 647)

Catalog No. ABIN1698960

This anti-GATA3 antibody is a Rabbit Polyclonal antibody detecting GATA3 in WB, IF (cc) and IF (p). Suitable for Mouse.

Quick Overview for GATA3 antibody (pSer162) (AbBy Fluor® 647) (ABIN1698960)

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA3 antibody is conjugated to AbBy Fluor® 647

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-GATA3 Antibody (AbBy Fluor® 647)

Binding Specificity: pSer162

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human GATA3 around the phosphorylation site of Ser162

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GATA3

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Alternative Name: GATA3

Background:

Synonyms: GATA3 phospho S162, p-GATA3 phospho S162, GATA3 phospho Ser162, p-GATA3 Ser162, GATA 3, GATA3, GATA-3, GATA binding factor 3, GATA binding protein 3, HDR, MGC2346, MGC5199, MGC5445, Trans acting T cell specic transcription factor GATA 3, GATA3_HUMAN, Trans-acting T-cell-specic transcription factor GATA-3, GATA-binding factor 3.

Background: Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as in erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. Both GATA-4 and GATA-6 are found in heart, pancreas and ovary, lung and liver tissues exhibit GATA-6, but not GATA-4 expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart. Although expression patterns of the various GATA transcription factors may overlap, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites.

Gene ID: 2625

Pathways: Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation