Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

POU2AF1 antibody (AA 1-100) (Biotin)

This Rabbit Polyclonal antibody specifically detects POU2AF1 in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human.
Catalog No. ABIN1700614

Quick Overview for POU2AF1 antibody (AA 1-100) (Biotin) (ABIN1700614)

Target

See all POU2AF1 Antibodies
POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Reactivity

  • 60
  • 20
  • 5
Human

Host

  • 66
  • 9
  • 2
Rabbit

Clonality

  • 63
  • 14
Polyclonal

Conjugate

  • 33
  • 6
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This POU2AF1 antibody is conjugated to Biotin

Application

  • 48
  • 27
  • 26
  • 26
  • 23
  • 18
  • 12
  • 6
  • 5
  • 4
  • 2
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Binding Specificity

    • 15
    • 14
    • 11
    • 6
    • 5
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    AA 1-100

    Predicted Reactivity

    Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

    Purification

    Purified by Protein A.

    Immunogen

    KLH conjugated synthetic peptide derived from human BOB1

    Isotype

    IgG
  • Application Notes

    WB 1:300-5000
    IHC-P 1:200-400
    IHC-F 1:100-500

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 μg/μL

    Buffer

    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C for 12 months.

    Expiry Date

    12 months
  • Target

    POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

    Alternative Name

    BOB1

    Background

    Synonyms: B cell Oct binding protein 1, B cell specic coactivator OBF 1, B cell specic coactivator OBF1, B-cell-specic coactivator OBF-1, BOB 1, BOB-1, OBF 1, OBF1, OBF1_HUMAN, OCA B, OCA-B, OCAB, OCT binding factor 1, OCT-binding factor 1, POU domain class 2 associating factor 1, POU domain class 2-associating factor 1, POU2AF1.

    Background: POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer from the OCT1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1. Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, ATTTGCAT, and the proteins that bind to it. This is a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. Oct-1 encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by Oct-2. A novel protein designated Bob 1 (B cell Oct binding protein 1), alternatively called OBF-1, specifically interacts with Oct-1 and Oct-2, enhancing their transcriptional efficacy. Bob 1 is expressed at highest levels in spleen and peripheral blood leukocytes and represents an Oct co-factor capable of conferring cell-specific activation of Oct-1 and Oct-2. Although having no intrinsic capacity for DNA binding, Bob 1 associates tightly with the octamer motif in the presence of Oct-1 and/or Oct-2. The gene which encodes Bob 1 maps to human chromosome 11q23.1.

    Gene ID

    5450
You are here:
Chat with us!