SKAP1 antibody (AA 101-200) (Biotin)

Catalog No. ABIN1701587

The Rabbit Polyclonal anti-SKAP1 antibody has been validated for ELISA, IHC (p) and IHC (fro). It is suitable to detect SKAP1 in samples from Rat.

Quick Overview for SKAP1 antibody (AA 101-200) (Biotin) (ABIN1701587)

Target: SKAP1 (Src Kinase Associated phosphoprotein 1 (SKAP1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKAP1 antibody is conjugated to Biotin

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SKAP1 Antibody (Biotin)

Binding Specificity: AA 101-200

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SKAP55

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for SKAP1

Target: SKAP1 (Src Kinase Associated phosphoprotein 1 (SKAP1))

Alternative Name: SKAP55

Background:

Synonyms: pp55, SCAP 1, SCAP1, SKAP 1, SKAP 55, SKAP-55, Skap1, SKAP1_HUMAN, SKAP55 adaptor protein, SRC family associated phosphoprotein 1, Src family-associated phosphoprotein 1, Src kinase associated phosphoprotein 1, SRC kinase associated phosphoprotein 55 kD, Src kinase associated phosphoprotein of 55 kDa, Src kinase-associated phosphoprotein 1, Src kinase-associated phosphoprotein of 55 kDa.

Background: Fyb (Fyn binding protein) and the anchoring proteins SKAP55 and SKAP55-R (SKAP55-related protein) associate with the tyrosine kinase p59fyn. SKAP55 and SKAP55-R bind to Fyb through their SH3 domains and function as substrates for p59Fyn in resting T cells. SKAP55 contains an N-terminal pleckstrin homology domain and a C-terminal SH3 domain binding motif of adjacent arginine and lysine residues followed by tandem tyrosines. SKAP55-R, similar in overall structure to SKAP55, contains a coiled-coil N-terminal domain. SKAP55 associates with SLAP-130, another component of the Fyn complex, which plays a role in the regulation of signaling events initiated by lymphocyte antigen receptors leading up to T cell activation. The human SKAP55 gene maps to chromosome 17q21.32 and encodes a 359 amino acid protein.