SKAP2 antibody (AA 1-100) (Cy3)

Catalog No. ABIN1702204

This Rabbit Polyclonal antibody specifically detects SKAP2 in WB, IF (cc) and IF (p). It exhibits reactivity toward Human.

Quick Overview for SKAP2 antibody (AA 1-100) (Cy3) (ABIN1702204)

Target: SKAP2 (Src Kinase Associated Phosphoprotein 2 (SKAP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKAP2 antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-SKAP2 Antibody (Cy3)

Binding Specificity: AA 1-100

Predicted Reactivity: Human,Mouse,Rat,Dog,Sheep,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SCAP2

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SKAP2

Target: SKAP2 (Src Kinase Associated Phosphoprotein 2 (SKAP2))

Alternative Name: Scap2

Background:

Synonyms: Fyn associated phosphoprotein SKAP55 homologue, MGC10411, MGC33, PRAP, Pyk2/RAFTK associated protein, Pyk2/RAFTK-associated protein, RA70, Retinoic acid-induced protein 70, SAPS, SKAP-55HOM, SKAP-HOM, skap2, SKAP2_HUMAN, SKAP55 homolog, SKAP55R, Src associated adaptor protein, Src family associated phosphoprotein 2, Src family-associated phosphoprotein 2, src kinase associated phosphoprotein of 55 related protein, Src kinase-associated phosphoprotein 2, Src kinase-associated phosphoprotein 55-related protein, Src-associated adapter protein with PH and SH3 domains.

Background: Fyb (Fyn binding protein) and the anchoring proteins SKAP55 and SKAP55-R (SKAP55-related protein) associate with the tyrosine kinase p59fyn. SKAP55 and SKAP55-R bind to Fyb through their SH3 domains and function as substrates for p59Fyn in resting T cells. SKAP55 contains an N-terminal pleckstrin homology domain and a C-terminal SH3 domain binding motif of adjacent arginine and lysine residues followed by tandem tyrosines (i.e. RKxxYxxY). SKAP55-R, similar in overall structure to SKAP55, contains a coiled-coil N-terminal domain. SKAP55 associates with SLAP-130, another component of the Fyn complex, which plays a role in the regulation of signaling events initiated by lymphocyte antigen receptors leading up to T cell activation. The human SKAP55 gene maps to chromosome 17q21.32 and encodes a 359 amino acid protein.