Spartan antibody (AA 31-130) (Cy3)

Catalog No. ABIN1702984

This Rabbit Polyclonal antibody specifically detects Spartan in IF (cc) and IF (p). It exhibits reactivity toward Human.

Quick Overview for Spartan antibody (AA 31-130) (Cy3) (ABIN1702984)

Target: Spartan (C1orf124) (Chromosome 1 Open Reading Frame 124 (C1orf124))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Spartan antibody is conjugated to Cy3

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Spartan Antibody (Cy3)

Binding Specificity: AA 31-130

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human C1orf124

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Spartan

Target: Spartan (C1orf124) (Chromosome 1 Open Reading Frame 124 (C1orf124))

Alternative Name: C1orf124

Background:

Synonyms: C1orf124, CA124_HUMAN, Chromosome 1 open reading frame 124, DDDL1880, dJ876B10.3, PRO4323, RP5-876B10.3, Zinc finger RAD18 domain-containing protein C1orf124, SPRTN, SprT-like domain-containing protein Spartan, DVC1, DNA damage protein targeting VCP.

Background: Regulator of UV-induced DNA damage response: acts as a 'reader' of ubiquitinated PCNA that enhances RAD18-mediated PCNA ubiquitination and translesion DNA synthesis (TLS). Recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA. Facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis. Acts as a regulator of TLS by recruiting VCP/p97 to sites of DNA damage, possibly leading to extraction of DNA polymerase eta (POLH) by VCP/p97 to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage.

Gene ID: 83932

UniProt: Q9H040