EED antibody (AA 51-150) (Cy5.5)

Catalog No. ABIN1706384

Product Details anti-EED Antibody (Cy5.5)

Target: EED (Embryonic Ectoderm Development (EED))

Binding Specificity: AA 51-150

Reactivity: Xenopus laevis, Killifish (Oryzias latipes), Xenopus tropicalis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EED antibody is conjugated to Cy5.5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Killifish (Oryzias latipes), Xenopus laevis, Xenopus tropicalis

Predicted Reactivity: Human,Mouse,Rat,Cow,Sheep,Pig,Horse,Chicken,Rabbit,Zebrafish

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human EED

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EED

Target: EED (Embryonic Ectoderm Development (EED))

Alternative Name: EED (EED Products)

Background:

Synonyms: eed, EED protein, EED_HUMAN, Embryonic ectoderm development, Embryonic ectoderm development isoform a, Embryonic ECTODERM development protein homolog, hEED, OTTHUMP00000235483, OTTHUMP00000235484, OTTHUMP00000235485, Polycomb protein eed, WAIT 1, WAIT-1, WAIT1, WD protein associating with integrin cytoplasmic tails 1.

Background: The transcriptional repressing Polycomb-group (PcG) and transcriptional activating trithorax-group (trxG) genes of Drosophila are part of a cellular memory system responsible for the stable inheritance of gene activity. PcG proteins assemble into multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. EED (embryonic ectoderm development) is the human homolog of Eed, a murine PcG gene homologous to the Drosophila homeotic gene, extra sex combs. The human EED protein is 99. 5 % identical to the mouse EED protein and contains seven WD repeats, which are involved in protein-protein interactions. There are two human EED transcripts that contain a putative 407-nucleotide-long intron and give rise to two HEED protein isoforms, 535 and 494 amino acids in length. EED interacts in a highly specific manner, both in vitro and in vivo, with histone deacetylase (HDAC) proteins.

Gene ID: 8726