Nemo-Like Kinase antibody (AA 101-200) (FITC)

Catalog No. ABIN1709710

This Rabbit Polyclonal antibody specifically detects Nemo-Like Kinase in WB, IF (cc) and IF (p). It exhibits reactivity toward Human.

Quick Overview for Nemo-Like Kinase antibody (AA 101-200) (FITC) (ABIN1709710)

Target: Nemo-Like Kinase (NLK)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nemo-Like Kinase antibody is conjugated to FITC

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Nemo-Like Kinase Antibody (FITC)

Binding Specificity: AA 101-200

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Cow,Sheep,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Nemo-like kinase

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Nemo-Like Kinase

Target: Nemo-Like Kinase (NLK)

Alternative Name: Nemo-like kinase

Background:

Synonyms: DKFZp761G1211, FLJ21033, LAK1, Nemo like kinase, Nemo-like kinase, Nlk, NLK_HUMAN, Protein LAK1, Serine/threonine protein kinase NLK, Serine/threonine-protein kinase NLK.

Background: Nemo-like kinase (NLK) plays a role in cell fate determination and is required for differentiation of bone marrow stromal cells. It acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. It may suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP. (referenced from swissprot)

Pathways: Ubiquitin Proteasome Pathway