Dnmt2 antibody (AA 61-160) (FITC)

Catalog No. ABIN1710699

This anti-Dnmt2 antibody is a Rabbit Polyclonal antibody detecting Dnmt2 in IF (cc) and IF (p). Suitable for Human.

Quick Overview for Dnmt2 antibody (AA 61-160) (FITC) (ABIN1710699)

Target: Dnmt2 (TRDMT1) (tRNA Aspartic Acid Methyltransferase 1 (TRDMT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dnmt2 antibody is conjugated to FITC

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Dnmt2 Antibody (FITC)

Binding Specificity: AA 61-160

Predicted Reactivity: Human,Mouse,Rat,Cow,Sheep,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Dnmt2

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Dnmt2

Target: Dnmt2 (TRDMT1) (tRNA Aspartic Acid Methyltransferase 1 (TRDMT1))

Alternative Name: Dnmt2

Background:

Synonyms: dDNMT, D mMT 2, D mMT2, DNA cytosine 5 methyltransferase 2, DNA cytosine 5 methyltransferase like protein 2, DNA cytosine-5-methyltransferase-like protein 2, DNA 5 cytosine methyltransferase, DNA methyltransferase 2, DNA methyltransferase homolog HsaIIP, DNA MTase homolog HsaIIP, Dnmt 2, Dnmt2, M.HsaIIP, MHsaIIP, nmt 2, nmt2, OTTHUMP00000045198, PuMet, RNMT 1, RNMT1, TRDMT 1, TRDMT_HUMAN, TRDMT1, tRNA cytosine 5 methyltransferase, tRNA cytosine38-C5-methyltransferase, tRNA aspartic acid methyltransferase 1, tRNA aspartic acid methyltransferase 1 variant 1, tRNA aspartic acid methyltransferase 1 variant 2, tRNA aspartic acid methyltransferase 1 variant 3, tRNA aspartic acid methyltransferase 1 variant 4, tRNA aspartic acid methyltransferase 1 variant 5, tRNA aspartic acid methyltransferase 1 variant 8.

Background: Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.

Gene ID: 1787