ATPase Inhibitory Factor 1 antibody (AA 26-106) (HRP)

Catalog No. ABIN1711933

This Rabbit Polyclonal antibody specifically detects ATPase Inhibitory Factor 1 in WB and ELISA. It exhibits reactivity toward Rat.

Quick Overview for ATPase Inhibitory Factor 1 antibody (AA 26-106) (HRP) (ABIN1711933)

Target: ATPase Inhibitory Factor 1 (ATPIF1)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATPase Inhibitory Factor 1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Product Details anti-ATPase Inhibitory Factor 1 Antibody (HRP)

Binding Specificity: AA 26-106

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ATPIF1/ATPase Inhibitory Factor 1

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ATPase Inhibitory Factor 1

Target: ATPase Inhibitory Factor 1 (ATPIF1)

Alternative Name: ATP1/ATPase Inhibitory Factor 1

Background:

Synonyms: AT1_HUMAN, ATP synthase inhibitor protein, ATPase inhibitor, ATPase inhibitor mitochondrial, ATPase inhibitor protein, ATPase inhibitory factor 1, ATPI, ATP 1, ATP1, ATPIP, 1, 1, Inhibitor of F1Fo-ATPase, IP, MGC1167, MGC8898, mitochondrial.

Background: Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core, and F0, the membrane-embedded protein channel. F1 consists of three Alpha chains and three Beta chains, which are weakly homologous, as well as one Gamma chain, one Delta chain and one Gamma chain. F0 consists of three subunits: a, b and c. A mitochondrial F1-ATPase inhibitor protein, ATPIF1 (ATPase inhibitory factor 1), also known as IP, IF1, ATPI or ATPIP (ATPase inhibitor protein), binds to the C-terminal region of a Beta subunit of the F1-ATPase at low pH values and, via interference of the Beta and Gamma subunit interaction, ATPIF1 regulates the activity of the F1F0-ATPase. This reversible ATPIF1 binding to F1F0-ATPase also occurs on the surface of endothelial cells.

Gene ID: 93974