BOLL antibody (AA 1-100) (HRP)

Catalog No. ABIN1712185

This Rabbit Polyclonal antibody specifically detects BOLL in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Sheep.

Quick Overview for BOLL antibody (AA 1-100) (HRP) (ABIN1712185)

Target: BOLL (Bol, Boule-Like (BOLL))

Reactivity: Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BOLL antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-BOLL Antibody (HRP)

Binding Specificity: AA 1-100

Cross-Reactivity: Sheep

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Monkey

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human BOULE

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for BOLL

Target: BOLL (Bol, Boule-Like (BOLL))

Alternative Name: BOULE

Background:

Synonyms: Bol Drosophila boule homolog like, Bol, Bol boule like, Bol, boule like Drosophila, bol, boule-like Drosophila, Boll, BOLL_HUMAN, BOULE, Boule like, BOULE, Drosophila, homolog of, Protein boule like, Protein boule-like, Putative uncharacterized protein BOLL.

Background: Spermatogenesis represents the intricate developmental process of mitotic and meiotic cell divisions that ultimately leads to the production of haploid spermatozoa. BOULE, a member of the human deleted in azoospermia (DAZ) family, functions as a key conserved switch that regulates the progression of germ cells through meiosis in man. BOULE is an RNA-binding protein that regulates the expression of twine, a Cdc25 phosphatase, which promotes progression through meiosis. BOULE is expressed not only in the testis, but also in the nervous system, where it may play a role in neural communication. Mutations in the BOULE gene are be associated with male infertility, and the relative proportions of the three BOULE isoforms (B1, B2 and B3) may function as predictive markers for meiotic efficiency.

Gene ID: 66037