Glucose Oxidase antibody (HRP)

Catalog No. ABIN1712648

The Rabbit Polyclonal anti-Glucose Oxidase antibody has been validated for ELISA, WB, IHC (fro) and IHC (p). It is suitable to detect Glucose Oxidase in samples from Others.

Quick Overview for Glucose Oxidase antibody (HRP) (ABIN1712648)

Target: Glucose Oxidase (GOD)

Reactivity: Others

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glucose Oxidase antibody is conjugated to HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Glucose Oxidase Antibody (HRP)

Cross-Reactivity (Details): Penicillium amagasakiense

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Penicillium amagasakiense Glucose Oxidase

Isotype: IgG

Application Details

Application Notes: WB 1:100-1000
IHC-P 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Glucose Oxidase

Target: Glucose Oxidase (GOD)

Alternative Name: Glucose Oxidase

Background:

Synonyms: Beta D Glucose Oxygen 1 Oxido Reductase, Glucose oxidase Precursor, Glucose Oxyhydrase, GOD, GOX_PENAG.

Background: Glucose Oxidase is a dimeric enzyme that binds to _-D-glucose and aids in its oxidation into D-glucono-1,5-lactone which then hydrolyzes to gluconic acid. Flavin adenine dinucleotide (FAD) is a cofactor to Glucose Oxidase that acts as the initial electron acceptor and is required for this oxidation to occur. Glucose Oxidase is a natural preservative found in honey, where it reduces atmospheric oxygen into hydrogen peroxide which acts as an antibacterial barrier. Glucose Oxidase is also commonly used in biosensors in which it conveys levels of glucose by keeping track of the number of electrons passed through the enzyme. In this application, Glucose Oxidase is connected to an electrode and the resulting charge is measured.