CXCR2 antibody (pSer347)

Catalog No. ABIN1713644

The Rabbit Polyclonal anti-CXCR2 antibody has been validated for WB, ELISA, ICC, IHC (p), IHC (fro), IF (p) and IF (cc). It is suitable to detect CXCR2 in samples from Mouse.

Quick Overview for CXCR2 antibody (pSer347) (ABIN1713644)

Target: CXCR2 (Chemokine (C-X-C Motif) Receptor 2 (CXCR2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCR2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-CXCR2 Antibody

Binding Specificity: pSer347

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Dog,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human CXCR2 around the phosphorylation site of Ser347

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CXCR2

Target: CXCR2 (Chemokine (C-X-C Motif) Receptor 2 (CXCR2))

Alternative Name: CXCR2

Background:

Synonyms: CXCR2 phospho S347, CXCR2 phospho Ser347, p-CXCR2 phospho S347, p-CD182 phospho Ser347, CXC-chemokine receptor 2, CD 182, CD182, CD182 antigen, CDw128b, Chemokine CXC receptor 2, CMKAR2, CXCR 2, CXC R2, CXC-R2, CXCR2_HUMAN, C-X-C chemokine receptor type 2, CXCR-2, GRO/MGSA receptor, High affinity interleukin-8 receptor B, IL-8R B, IL-8 receptor type 2.

Background: The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009].

Gene ID: 3579

Pathways: cAMP Metabolic Process