ADH5 antibody (AA 301-374)

Catalog No. ABIN1713695

The Rabbit Polyclonal anti-ADH5 antibody has been validated for ELISA, FACS, IF (cc), IF (p), IHC (p), ICC and IHC (fro). It is suitable to detect ADH5 in samples from Mouse.

Quick Overview for ADH5 antibody (AA 301-374) (ABIN1713695)

Target: ADH5 (Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADH5 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ADH5 Antibody

Binding Specificity: AA 301-374

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ADH5

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ADH5

Target: ADH5 (Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5))

Alternative Name: ADH5

Background:

Synonyms: ADH 3, ADH5, ADHX, ADHX_HUMAN, Alcohol dehydrogenase class III chi polypeptide, alcohol dehydrogenase 5 class III chi polypeptide, Alcohol dehydrogenase 5, Alcohol dehydrogenase class 3, Alcohol dehydrogenase class chi chain, Alcohol dehydrogenase class III, Alcohol dehydrogenase class-3, Alcohol dehydrogenase class-III, class III alcohol dehydrogenase 5 chi subunit, FALDH, FDH, formaldehyde dehydrogenase, Glutathione dependent formaldehyde dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, GSH-FDH, hydroxymethyllutathione dehydrogenase, S-hydroxymethylglutathione dehydrogenase.

Background: The alcohol dehydrogenase family of proteins metabolize a wide variety of substrates, including retinol, hydroxysteroids, ethanol, aliphatic alcohols and lipid peroxidation products. ADH5 (alcohol dehydrogenase 5 (class III)), also known as FDH (formaldehyde dehydrogenase), ADHX, ADH-3 or GSNOR, is a 374 amino acid cytoplasmic protein that belongs to the class III subfamily of alcohol dehydrogenases. Expressed ubiquitously, ADH5 uses iron as a cofactor to catalytically oxidize both long-chain primary alcohols and S-hydroxymethyl-glutathione, a product formed spontaneously between formaldehyde and glutathione. ADH5 exists as a homodimer and, via its ability to oxidize S-hydroxymethyl-glutathione and, thus, eliminate formaldehyde, functions as an important component of cellular metabolism. Genetic variations in the gene encoding ADH5 may affect drug and alcohol dependence in humans.

Gene ID: 128

UniProt: P11766