ABL1 antibody (pTyr204)

Catalog No. ABIN1714406

The Rabbit Polyclonal anti-ABL1 antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (p), ICC and IHC (fro). It is suitable to detect ABL1 in samples from Mouse and Rat.

Quick Overview for ABL1 antibody (pTyr204) (ABIN1714406)

Target: ABL1 (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ABL1 Antibody

Binding Specificity: pTyr204

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Cow,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human c-Abl isoform b around the phosphorylation site of Tyr204

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ABL1

Target: ABL1 (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1))

Alternative Name: c-ABL

Background:

Synonyms: c-Ablphospho Y204, c-Ablphospho Tyr204, p-c-AblTyr204, Abelson Murine Leukemia Viral Oncogene Homolog 1, Abelson murine leukemia viral v abl oncogene homolog 1, Abl 1, ABL, Abl protein, Abl1, Bcr/c abl oncogene protein, JTK 7, JTK7, p150, Proto oncogene tyrosine protein kinase ABL1, Transformation gene oncogene ABL, v abl Abelson murine leukemia viral oncogene homolog 1, v abl, ABL1_HUMAN.

Background: The c Abl proto oncogene encodes a protein tyrosine kinase that is located in the cytoplasm and nucleus. In chronic myelogenous leukemia and in a subset of acute lymphoblastic leukemias, the c Abl proto oncogene undergoes a (9,22) chromosomal translocation producing a novel rearranged chromosome (the Philadelphia chromosome) As the result of the fusion of c Abl sequences from chromosome 9 to the Bcr gene on chromosome 22. The molecular consequence of this translocation is the generation of a chimeric Bcr/Abl mRNA encoding activated Abl protein tyrosine kinase.

Gene ID: 25

Pathways: Apoptosis, Regulation of Muscle Cell Differentiation, Platelet-derived growth Factor Receptor Signaling, Lipid Metabolism