AGO5 antibody
This Rabbit Polyclonal antibody specifically detects AGO5 in IP and WB. It exhibits reactivity toward Arabidopsis thaliana.
Catalog No. ABIN1720795
Quick Overview for AGO5 antibody (ABIN1720795)
Target
AGO5
(Argonaute Family Protein (AGO5))
Reactivity
Arabidopsis thaliana
Host
Rabbit
Clonality
Polyclonal
Application
Immunoprecipitation (IP), Western Blotting (WB)
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Specificity
- TAIR: At2g27880
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Cross-Reactivity (Details)
- Not reactive in: Zea mays
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Predicted Reactivity
- Arabidopsis thaliana
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Purification
- serum
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Immunogen
- peptide of Arabidopsis thaliana AGO5 UniProt: Q9SJK3, TAIR:AT2G27880
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Application Notes
- 1: 1500 (WB), 5 μg per gram floral tissue (IP)
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Comment
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AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 200 μL of sterile water.
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Handling Advice
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Avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. -
Storage Comment
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- AGO5 (Argonaute Family Protein (AGO5))
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Alternative Name
- AGO5
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Background
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AGI Code: AT2G27880
AGO5 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). Probably involved in antiviral RNA silencing. -
Molecular Weight
- expected: 111 kDa, appearent: 111 kDa
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UniProt
- Q9SJK3
Target
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