NpHR antibody
-
- Target
- NpHR
- Reactivity
- Natronomonas pharaonis (Natronobacterium pharaonis)
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Immunofluorescence (IF), Western Blotting (WB)
- No Cross-Reactivity
- Halobacterium salinarum
- Cross-Reactivity (Details)
- Not reactive in: Halobacterium salinarum (HsHR and HsBR)
- Predicted Reactivity
- Natronomonas pharaonis
- Purification
- serum
- Immunogen
- KLH-conjugated synthetic peptide derived from Natronomonas pharaonis halorhodopsin sequences Q3ITX1
-
- Application Notes
- 1 : 1000 - 1: 20 000 with ECL (WB), 1: 200 (IF)
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 μL of sterile water.
- Handling Advice
-
Avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. - Storage
- -20 °C
- Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
-
- Target
- NpHR
- Background
- Halorhodopsin (HR) is a hyperpolarizing light-driven ion pump from the halophilic archaea bacterium Natronomonas pharaonis. HR uses the energy of yellow light (excitation maximum near 580 nm) to mediate primarily chloride but also bromide, iodide, and nitrate import into the cell against their electrochemical gradients.
- Molecular Weight
- expected: 30 kDa, appearent: 30 kDa
- UniProt
- Q3ITX1
-