Actin antibody

Catalog No. ABIN1720863

Cited in 1 publication. The Rabbit Polyclonal anti-Actin antibody (ABIN1720863) specifically detects Actin in WB, IF and ExM. The antibody is reactive with Nicotiana tabacum, Barley, Arabidopsis thaliana, Cyanthobasis fruticulosa, Zea mays, Vitis vinifera, Vigna unguiculata, Triticum aestivum, Soybean, Setaria italica, Salsola tragus, Salsola grandis, Rape, Potato, Picrorhiza kurroa, Phoenix dactylifera, Phaeodactylum tricornutum, Petrosimonia nigdeensis, Odontarrhena lesbiaca, Kiwi, Green bean, Fragaria x ananassa, Cynara cardunculus, Cucumis sativus and Agrostis stolonifera samples.

Quick Overview for Actin antibody (ABIN1720863)

Target: Actin

Reactivity: Nicotiana tabacum, Barley, Arabidopsis thaliana, Cyanthobasis fruticulosa, Zea mays, Vitis vinifera, Vigna unguiculata, Triticum aestivum, Soybean, Setaria italica, Salsola tragus, Salsola grandis, Rape, Potato, Picrorhiza kurroa, Phoenix dactylifera, Phaeodactylum tricornutum, Petrosimonia nigdeensis, Odontarrhena lesbiaca, Kiwi, Green bean, Fragaria x ananassa, Cynara cardunculus, Cucumis sativus, Agrostis stolonifera

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Expansion Microscopy (ExM)

Product Details anti-Actin Antibody

Purpose: Anti-ACT | Actin (polyclonal)

Specificity: TAIR: At2g37620, At2g18780, At2g53750, At2g59370, At2g42100, At5g09810, At2g49240, At2g12110, At2g46520

Cross-Reactivity (Details): Actinidia sp., Agostis stoloniferacv. 'Penncross', Arabidopsis thaliana, Brassica napus, Cucumis sativus, Cyanthobasis fruticulosa, Cynara cardunculus, Fragaria x ananassa, Glycine max, Hordeum vulgare, Nicotiana tabacum, Odontarrhena lesbiaca, Petrosimonia nigdeensis, Phaseolus vulgaris, Phaeodactylum tricornutum, Phoenix dactylifera, Picrorhiza kurroa,Salsola grandis, Salsola tragus, Setaria italica, Solanum tuberosum, Triticum aestivum, Vigna unguiculata, Vitis vinifera, Zea mays
Not reactive in: Chlamydomonas reinhardtii (too high background for this species)

Predicted Reactivity: Agropyron cristatum,Beta vulgaris,Betula luminifera,Brassica rapa subsp. pekinensis,Daucus carota,Cannabis sativa L.,Capsella rubella,Capsicum annuum,Castanea sativa,Chorispora bungeana,Cyanidioschyzon merolae strain 10D,Glycine soja,Halogeton glomeratus,Helianthus annuus,Ipomoea batatas,Manihot esculenta,Medicago truncatula,Malus domestica,Oryza sativa,Pisum sativun,Populus sp.,Saccharum officinarum,Solanum lycopersicum,Solanum tuberosum,Phaeodactylum tricornutum,Picea abies,Picea sitchensis,Prunus avium,Olea europaea,Ricinus communis,Rubus plicatus,Theobroma cacao,Trebouxia sp.,Vicia faba

Purification: Serum

Immunogen: ca. 100 amino acids of recombinant actin conserved more than 80% in Arabidopsis thaliana: actin-1 P0CJ46 AT2G37620

Application Details

Application Notes: 1: 250 (ExM), 1-100 - 1 : 250 (IF), 1 : 3000-1 : 5000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-Actin antibody (ABIN1720863)

Zheng, Fang, Pan, Sun, Deng, Grosser, Guo: "iTRAQ-based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo." in: Journal of proteome research, Vol. 13, Issue 6, pp. 2998-3015, (2014) (PubMed).

Target Details for Actin

Target: Actin

Alternative Name: Actin (ACT)

Background:

Background: Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.

Additional information: Antibody available in 3 various pack sizes: 50, 100 and 150 μL - Please inquire.This product can be sold containing ProClin if requested.

Molecular Weight: 41.6 | 45 kDa

Gene ID: 818339

UniProt: P0CJ46, Q96292, P0CJ47, P53494, Q8RYC2, P53492, Q96293, P53496, P53497