Actin antibody

Catalog No. ABIN1720863

This anti-Actin antibody is a Rabbit Polyclonal antibody detecting Actin in WB and IF. Suitable for Barley, Arabidopsis thaliana, Soybean and Zea mays. This Primary Antibody has been cited in 1 publication.

Quick Overview for Actin antibody (ABIN1720863)

Target: Actin

Reactivity: Barley, Arabidopsis thaliana, Soybean, Zea mays

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-Actin Antibody

Specificity: TAIR: At2g37620, At2g18780, At2g53750, At2g59370, At2g42100, At5g09810, At2g49240, At2g12110, At2g46520

Cross-Reactivity (Details): Not reactive in: Chlamydomonas reinhardtii (too high background for this species)

Predicted Reactivity: Agropyron cristatum, Beta vulgaris, Betula luminifera, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Castanea sativa, Chorispora bungeana, Cucumis sativus, Cyanidioschyzon merolae strain 10D, Glycine max, Glycine soja, Halogeton glomeratus, Medicago truncatula, Malus domestica, Nicotiana tabacum, Oryza sativa, Pisum sativun, Solanum lysopersicum, Solanum tuberosum, Phaseolus vulgaris, Picea abies, Picea sitchensis, Prunus avium, Ricinus communis, Rubus plicatus, Theobroma cacao, Triticum aestivum, Vicia faba

Purification: serum

Immunogen: ca. 100 amino acids of recombinant actin conserved more than 80 % in Arabidopsis thaliana: actin-1 P0CJ46 AT2G37620, actin-2 Q96292 AT3G18780, actin-3 P0CJ47 AT3G53750, actin-4 P53494 AT5G59370, actin-5 Q8RYC2 At2g42100, actin-7 P53492 At5g09810, actin-8 Q96293 AT1G49240 , actin-11 P53496 , AT3G12110 ,actin-12 P53497 AT3G46520

Application Details

Application Notes: 1 : 2500 with standard ECL (WB), 1: 250 (IF)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Handling Advice: Avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References for anti-Actin antibody (ABIN1720863)

Zheng, Fang, Pan, Sun, Deng, Grosser, Guo: "iTRAQ-based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo." in: Journal of proteome research, Vol. 13, Issue 6, pp. 2998-3015, (2014) (PubMed).

Target Details for Actin

Target: Actin

Alternative Name: Actin (ACT)

Background: Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.

Molecular Weight: expected: 41.6 kDa, appearent: 45 kDa

UniProt: P0CJ46, Q96292, P0CJ47, P53494, Q8RYC2, P53492, Q96293, P53496, P53497