AGO2 antibody

Catalog No. ABIN1720882

This Rabbit Polyclonal antibody specifically detects AGO2 in WB. It exhibits reactivity toward Arabidopsis thaliana.

Quick Overview for AGO2 antibody (ABIN1720882)

Target: AGO2 (Argonaute 2 (AGO2))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGO2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AGO2 Antibody

Specificity: TAIR: At1g48410

Cross-Reactivity (Details): Not reactive in: Chlamydomonas reinhardtii, Solanum lycopersicum, Zea mays

Predicted Reactivity: Capsella rubella, Solanum tuberosum

Purification: serum

Immunogen: KLH-conjugated synthetic peptide, derived from Arabidopsis thaliana AGO2 protein, UniProt: Q9SHF3, TAIR: AT1G31280

Application Details

Application Notes: 1: 250 - 1: 500 (WB)

Comment:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Handling Advice: Avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for AGO2

Target: AGO2 (Argonaute 2 (AGO2))

Alternative Name: AGO2

Background: AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Molecular Weight: expected: 116.4 kDa, appearent: 130 kDa

UniProt: Q9SHF3

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization