CD81 antibody

Catalog No. ABIN1720914

This anti-CD81 antibody is a Rat Monoclonal antibody detecting CD81 in FACS and cELISA. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for CD81 antibody (ABIN1720914)

Target: CD81

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This CD81 antibody is un-conjugated

Application: Flow Cytometry (FACS), Cell-ELISA (cELISA)

Clone: QV-6A8-S3

Product Details anti-CD81 Antibody

Specificity: Anti-human CD81 (TAPA-1)

Characteristics: Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells

Purification: Protein G

Immunogen: genetic immunisation with cDNA encoding human CD81

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry: 1.2 µg/10^6 cells
ELISA: 1:200 - 1:400
CELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.

Comment:

Synonyms: TAPA-1

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: short term: 2 °C - 8 °C, long term: -20 °C

References for anti-CD81 antibody (ABIN1720914)

Fofana, Xiao, Thumann, Turek, Zona, Tawar, Grunert, Thompson, Zeisel, Baumert: "A novel monoclonal anti-CD81 antibody produced by genetic immunization efficiently inhibits Hepatitis C virus cell-cell transmission." in: PLoS ONE, Vol. 8, Issue 5, pp. e64221, (2013) (PubMed).

Target Details for CD81

Target: CD81

Alternative Name: CD81

Background: CD81 (TAPA-1) belongs to the transmembrane 4 superfamily (tetraspanin family). CD81 is a widely expressed cell-surface protein that is characterized by the presence of four transmembranic domains, short N and C termini, a small extracellular loop (SEL) and a large extra-cellular loop (LEL). It is expressed on cells of hematopoietic, neuroectodermal and mesenchymal origin and plays a role in the regulation of cell development, cell-growth and signal transduction. CD81 plays a critical role in Hepatitis C Infection and is involved in HCV entry due to its ability to interact with virus' E1/E2 glycoproteins. CD81 is an essential HCV host factor as silencing of CD81 expression by CD81-specific monoclonal antibodies in hepatoma cells inhibits HCV entry.

UniProt: P60033

Pathways: Inositol Metabolic Process, Hepatitis C