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Ubiquitin antibody

This Mouse Monoclonal antibody specifically detects Ubiquitin in WB and ELISA. It exhibits reactivity toward Human, Mouse, Rat and Cow.
Catalog No. ABIN1721722

Quick Overview for Ubiquitin antibody (ABIN1721722)

Target

See all Ubiquitin (Ub) Antibodies
Ubiquitin (Ub)

Reactivity

  • 175
  • 72
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  • 66
  • 20
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  • 1
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  • 1
Human, Mouse, Rat, Cow

Host

  • 108
  • 92
  • 3
  • 1
  • 1
Mouse

Clonality

  • 112
  • 93
Monoclonal

Conjugate

  • 84
  • 15
  • 11
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  • 7
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  • 3
  • 3
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  • 3
  • 3
  • 3
  • 1
  • 1
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  • 1
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  • 1
  • 1
  • 1
  • 1
  • 1
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  • 1
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This Ubiquitin antibody is un-conjugated

Application

  • 179
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Western Blotting (WB), ELISA

Clone

5B9-B3
  • Specificity

    Reacts with human Ubiquitin. Cross-reacts with mouse, rat and bovine protein.

    Purification

    Purified (protein G)

    Immunogen

    Native bovine ubiquitin
  • Application Notes

    Working dilution: Optimal dilution should be determined by the end user.
    The following are guidelines only :
    WB 1 μg/mL. ELISA 1 μg/mL.

    Positive control: HeLa cell lysate

    Restrictions

    For Research Use only
  • Buffer

    PBS, pH 7.4,

    Handling Advice

    Dilute in PBS or medium which is identical to that used in the assay system.

    Storage

    4 °C/-20 °C

    Storage Comment

    Short term storage at+4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 months from date of receipt.

    Expiry Date

    6 months
  • Target

    Ubiquitin (Ub)

    Alternative Name

    Ubiquitin

    Background

    Conjugation of ubiquitin monomers or polymers can lead to various effects within a cell, depending on the residues to which ubiquitin is conjugated. Ubiquitination has been associated with protein degradation, DNA repair, cell cycle regulation, kinase modification, endocytosis, and regulation of other cell signaling pathways.

    Gene name: UBC

    Gene ID

    7316

    UniProt

    P0CG48

    Pathways

    Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
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