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CD73 antibody (AA 30-250)

Name: CD73 antibody (AA 30-250)
Brand: antibodies-online
SKU: ABIN1724945
Price: 533.5 USD
Availability: InStock

The Mouse Monoclonal anti-CD73 antibody has been validated for WB, IHC and ELISA. It is suitable to detect CD73 in samples from Human. There is 1 publication available.

Western blot analysis using NT5E mouse mAb against A431 (1) cell lysate. (CD73 antibody (AA 30-250))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (CD73 antibody (AA 30-250))

ELISA

Western blot analysis using NT5E mAb against human NT5E (AA: ) recombinant protein. (CD73 antibody (AA 30-250))

Western blot analysis using NT5E mAb against HEK293 (1) and NT5E (AA: 30-250)-hIgGFc transfected HEK293 (2) cell lysate. (CD73 antibody (AA 30-250))

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using NT5E mouse mAb with DAB staining. (CD73 antibody (AA 30-250))

IHC

Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues using NT5E mouse mAb with DAB staining. (CD73 antibody (AA 30-250))

IHC

6 images

(1 reference)

Catalog No. ABIN1724945

Datasheet as PDF

Quick Overview for CD73 antibody (AA 30-250) (ABIN1724945)

Target

See all CD73 (NT5E) Antibodies

CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity

Human

Host

Mouse

Clonality

Monoclonal

Conjugate

This CD73 antibody is un-conjugated

Application

Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone

4G6B10

Discover our top product NT5E Primary Antibody

Binding Specificity
16

13

9

9

8

6

5

5

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 30-250

Purpose

NT5E Antibody

Purification

Purified antibody

Immunogen

Purified recombinant fragment of human NT5E (AA: 30-250) expressed in E. Coli.

Isotype

IgG1
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 520-550), (C-Term) antibody
NT5E Reactivity: Human, Mouse WB, IHC (p) Host: Rabbit Polyclonal RB02846 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-500) antibody
NT5E Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 290-550) antibody
NT5E Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibody
NT5E Reactivity: Human WB, FACS, IHC, ELISA Host: Mouse Monoclonal 4G6E3 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody
NT5E Reactivity: Human WB, FACS, IHC, IF, StM Host: Mouse Monoclonal NT5E-2503 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody
NT5E Reactivity: Human WB, FACS, IHC, ELISA, IF, StM, Coat Host: Mouse Monoclonal NT5E-2545 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 48-527) antibody
NT5E Reactivity: Cow WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody (PE)
NT5E Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal AD2 PE

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-500) antibody
NT5E Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal D1 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-550) antibody
NT5E Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA: 1/10000

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Supernat, Markiewicz, Welnicka-Jaskiewicz, Seroczynska, Skokowski, Sejda, Szade, Czapiewski, Biernat, Zaczek: "CD73 expression as a potential marker of good prognosis in breast carcinoma." in: Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry, Vol. 20, Issue 2, pp. 103-7, (2012) (PubMed).
Target

CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name

NT5E

Background

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight

63.4 kDa

UniProt

P21589

Pathways

Synaptic Membrane, Ribonucleoside Biosynthetic Process