ERK1/2 antibody

Catalog No. ABIN1734127

This Rabbit Polyclonal antibody specifically detects ERK1/2 in WB. It exhibits reactivity toward Human, Mouse, Chicken, Xenopus laevis, Mammalian and Drosophila melanogaster.

Quick Overview for ERK1/2 antibody (ABIN1734127)

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reactivity: Human, Mouse, Chicken, Xenopus laevis, Mammalian, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1/2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ERK1/2 Antibody

Specificity: Reacts with human Erk1 and Erk2. Cross-reacts with mouse, rat, chicken, sheep, bovine, drosophila and xenopus protein.

Purification: Serum

Immunogen: 35 amino acid synthetic peptide corresponding to ERK1 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes: Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
WB1:5000

Positive control: HeLa cell

Restrictions: For Research Use only

Handling

Handling Advice: Dilute in PBS or medium which is identical to that used in the assay system.

Storage: 4 °C/-20 °C

Storage Comment: Short term storage at+4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 months from date of receipt.

Expiry Date: 6 months

Target Details for ERK1/2

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Alternative Name: Erk1 + Erk2

Background: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors.

Mitogen-activated protein kinase 3 & Mitogen-activated protein kinase 1 Extracellular signal-regulated kinase 1 & Extracellular signal-regulated kinase 3 ERK-1 & ERK-2 Gene name: MAPK3, MAPK1

UniProt: P27361, P28482