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GFAP antibody (AA 411-422) (FITC)

Name: GFAP antibody (AA 411-422) (FITC)
Brand: antibodies-online
SKU: ABIN1740957
Price: 784.88 USD
Availability: InStock

GFAP Reactivity: Human WB, IHC, IF, ICC Host: Mouse Monoclonal S206A-8 FITC

Western Blot analysis of Rat Brain Membrane showing detection of GFAP protein using Mouse Anti-GFAP Monoclonal Antibody, Clone S206A-8 . (GFAP antibody (AA 411-422) (FITC))

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GFAP Monoclonal Antibody, Clone S206A-8 (ABIN1740957). (GFAP antibody (AA 411-422) (FITC))

ICC

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GFAP Monoclonal Antibody, Clone S206A-8 . (GFAP antibody (AA 411-422) (FITC))

IF/ICC

3 images

Catalog No. ABIN1740957

Datasheet as PDF

Target See all GFAP Antibodies

GFAP (Glial Fibrillary Acidic Protein (GFAP))
Binding Specificity
33

27

20

16

12

9

9

8

8

7

7

6

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 411-422
Reactivity
289

172

170

51

51

34

31

11

11

10

7

6

5

4

3

2

2

2

1

1

1

1
Human
Host
190

140

12

2

2

2
Mouse
Compatible Secondaries
Clonality
190

157

1
Monoclonal
Conjugate
168

27

22

17

11

6

6

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1
This GFAP antibody is conjugated to FITC
Application
272

129

94

77

75

58

47

28

26

26

22

19

13

8

8

4

4

3

2

2

2

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity

Detects ~50 kDa. Cross-reacts with GFAP-R416W and other GFAP mutant proteins.

Cross-Reactivity

Human, Mouse, Rat

Purification

Protein G Purified

Immunogen

Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP, 100% identical in rat and mouse

Clone

S206A-8

Isotype

IgG1

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GFAP Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

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GFAP Reactivity: Mouse WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated

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GFAP Reactivity: Rat WB, IHC, ICC, IP Host: Mouse Monoclonal C9 unconjugated

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GFAP Reactivity: Human WB, IHC, IF, ELISA, FACS Host: Mouse Monoclonal 1C91F1 unconjugated

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GFAP Reactivity: Human, Rat, Mouse WB, IHC, IF, ELISA, ICC Host: Rabbit Polyclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (C-Term) antibody
Verified GFAP Reactivity: Human, Rat, Mouse WB, ELISA Host: Goat Polyclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) antibody
GFAP Reactivity: Human WB, IHC, IF Host: Rabbit Monoclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (AA 108-211) antibody
GFAP Reactivity: Mouse WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated

Application Notes
- WB (1:1000)
- optimal dilutions for assays should be determined by the user.
Comment

1 μg/ml of ABIN1740957 was sufficient for detection of GFAP in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Storage Comment

Conjugated antibodies should be stored at 4°C
Target

GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name

GFAP (GFAP Products)

Background

The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proiliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia.

Gene ID

2670

NCBI Accession

NP_001124491

UniProt

P14136