VDAC1 antibody (AA 1-283) (APC)

Catalog No. ABIN1741225

This Mouse Monoclonal antibody specifically detects VDAC1 in WB, IHC, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for VDAC1 antibody (AA 1-283) (APC) (ABIN1741225)

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VDAC1 antibody is conjugated to APC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: S152B-23

Product Details anti-VDAC1 Antibody (APC)

Binding Specificity: AA 1-283

Specificity: Detects ~30 kDa. Does not cross-react with VDAC2 or VDAC3 (based on KO validation results).

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 1-283 (full-length) of human VDAC1. Mouse: 98% identity (279/283 amino acids identical). Rat: 98% identity (279/283 amino acids identical) >60% identity with VDAC2 and VDAC3.

Isotype: IgG2a

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN1741225 was sufficient for detection of VDAC1 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for VDAC1

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Alternative Name: VDAC1

Background: Voltage-dependent anion-selective channel protein 1 (also known as VDAC, VDAC1 or outer mitochondrial membrane protein porin 1) is the the outer mitochondrial membrane receptor for hexokinase and BCL2L1. VDAC forms a channel through the mitochondrial membrane and is involved in small molecule diffusion, cell volume regulation and apoptosis. VDAC may participate in the formation of the permeability transition pore complex (PTPC), which is responsible for the release of mitochondrial products that triggers apoptosis.

Gene ID: 7416

UniProt: P21796