SYT2 antibody (Lumenal Region)

Catalog No. ABIN1742203

This Rabbit Polyclonal antibody specifically detects SYT2 in WB, IP and ICC. It exhibits reactivity toward Rat and Mouse.

Quick Overview for SYT2 antibody (Lumenal Region) (ABIN1742203)

Target: SYT2 (Synaptotagmin II (SYT2))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-SYT2 Antibody

Binding Specificity: AA 1-11, Lumenal Region

Specificity: Specific for synaptotagmin 2.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide (aa 1-11 in mouse synaptotagmin 2) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 100
IHC: not tested yet

Comment:

These antibodies can be used for labeling of recycling synaptic vesicles by adding to living neurons or as a marker for exocytosis in isolated nerve terminals.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Target Details for SYT2

Target: SYT2 (Synaptotagmin II (SYT2))

Alternative Name: Synaptotagmin 2

Pathways: Synaptic Vesicle Exocytosis