SYN2 antibody (AA 440-458)

Catalog No. ABIN1742207

The Mouse Monoclonal anti-SYN2 antibody has been validated for WB and ICC. It is suitable to detect SYN2 in samples from Mouse, Human and Rat. There is 1 publication available.

Quick Overview for SYN2 antibody (AA 440-458) (ABIN1742207)

Target: SYN2 (Synapsin II (SYN2))

Reactivity: Mouse, Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SYN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Clone: 27E3

Product Details anti-SYN2 Antibody

Binding Specificity: AA 440-458

Specificity: Specific for synapsin 2

Purification: purified IgG

Immunogen: Synthetic peptide (aa 440-458 of mouse synapsin 2) coupled to KLH via an N-terminal added cysteine.

Isotype: IgG1

Application Details

Application Notes: WB: 1 : 500 up to 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500
IHC: not tested yet

Comment:

WB: This antibody is less sensitive than the polyclonal rabbit antibody. Enriched synaptic vesicles are recommended for westernblotting.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS.

Buffer: PBS, 0.02% sodium azide (= 0.02 µg)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-SYN2 antibody (ABIN1742207)

Acuna, Liu, Südhof: "How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs." in: Neuron, Vol. 91, Issue 4, pp. 792-807, (2016) (PubMed).

Target Details for SYN2

Target: SYN2 (Synapsin II (SYN2))

Alternative Name: Synapsin 2