Syntaxin 4 antibody (Cytoplasmic Domain)

Catalog No. ABIN1742220

This anti-Syntaxin 4 antibody is a Mouse Monoclonal antibody detecting Syntaxin 4 in WB, IP and ICC. Suitable for Rat and Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for Syntaxin 4 antibody (Cytoplasmic Domain) (ABIN1742220)

Target: Syntaxin 4 (STX4)

Reactivity: Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Syntaxin 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 139-2

Product Details anti-Syntaxin 4 Antibody

Binding Specificity: AA 1-273, Cytoplasmic Domain

Specificity: Specific for syntaxin 4.

Purification: purified IgG

Immunogen: Recombinant cytoplasmic domain of rat syntaxin 4 (aa 1-273).

Isotype: IgG1

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 100
IHC: not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-Syntaxin 4 antibody (ABIN1742220)

Milovanovic, Honigmann, Koike, Göttfert, Pähler, Junius, Müllar, Diederichsen, Janshoff, Grubmüller, Risselada, Eggeling, Hell, van den Bogaart, Jahn: "Hydrophobic mismatch sorts SNARE proteins into distinct membrane domains." in: Nature communications, Vol. 6, pp. 5984, (2015) (PubMed).

Target Details for Syntaxin 4

Target: Syntaxin 4 (STX4)

Alternative Name: Syntaxin 4

Pathways: Synaptic Vesicle Exocytosis