Syntaxin 4 antibody (Cytoplasmic Domain)

Catalog No. ABIN1742221

This Rabbit Polyclonal antibody specifically detects Syntaxin 4 in WB, IP and ICC. It exhibits reactivity toward Human, Rat, Mouse and Hamster and has been mentioned in 6+ publications.

Quick Overview for Syntaxin 4 antibody (Cytoplasmic Domain) (ABIN1742221)

Target: Syntaxin 4 (STX4)

Reactivity: Human, Rat, Mouse, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Syntaxin 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Syntaxin 4 Antibody

Binding Specificity: AA 1-273, Cytoplasmic Domain

Specificity: Specific for syntaxin 4.

Purification: antiserum

Immunogen: Recombinant cytoplasmic domain of rat syntaxin 4 (aa 1-273).

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 5000 (AP staining)
ICC: 1 : 100
IHC: not tested yet

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-Syntaxin 4 antibody (ABIN1742221)

Arnold, Adhikari, Kang, Xu 徐昊: "Munc18a clusters SNARE-bearing liposomes prior to trans-SNARE zippering." in: The Biochemical journal, Vol. 474, Issue 19, pp. 3339-3354, (2017) (PubMed).

Arasaki, Roy: "Legionella pneumophila promotes functional interactions between plasma membrane syntaxins and Sec22b." in: Traffic (Copenhagen, Denmark), Vol. 11, Issue 5, pp. 587-600, (2010) (PubMed).

Bajohrs, Darios, Peak-Chew, Davletov: "Promiscuous interaction of SNAP-25 with all plasma membrane syntaxins in a neuroendocrine cell." in: The Biochemical journal, Vol. 392, Issue Pt 2, pp. 283-9, (2005) (PubMed).

Li, Alexander, Schwartz: "Syntaxin isoform specificity in the regulation of renal H+-ATPase exocytosis." in: The Journal of biological chemistry, Vol. 278, Issue 22, pp. 19791-7, (2003) (PubMed).

Toyomura, Murata, Yamamoto, Oka, Sun-Wada, Wada, Futai: "From lysosomes to the plasma membrane: localization of vacuolar-type H+ -ATPase with the a3 isoform during osteoclast differentiation." in: The Journal of biological chemistry, Vol. 278, Issue 24, pp. 22023-30, (2003) (PubMed).

Riedel, Antonin, Fernandez-Chacon, Alvarez de Toledo, Jo, Geppert, Valentijn, Valentijn, Jamieson, Südhof, Jahn: "Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules." in: Molecular and cellular biology, Vol. 22, Issue 18, pp. 6487-97, (2002) (PubMed).

Target Details for Syntaxin 4

Target: Syntaxin 4 (STX4)

Alternative Name: Syntaxin 4

Pathways: Synaptic Vesicle Exocytosis