NAPG antibody (AA 2-185)

Catalog No. ABIN1742244

This anti-NAPG antibody is a Rabbit Polyclonal antibody detecting NAPG in WB and IP. Suitable for Human, Mouse, Rat and Cow. This Primary Antibody has been cited in 2+ publications.

Quick Overview for NAPG antibody (AA 2-185) (ABIN1742244)

Target: NAPG (N-Ethylmaleimide-Sensitive Factor Attachment Protein, gamma (NAPG))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAPG antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-NAPG Antibody

Binding Specificity: AA 2-185

Specificity: Specific for gamma SNAP.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide AAQKINEGLEHLAKAEK (2-18 in rat gamma SNAP) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 500 up to 1 : 2000 (AP staining)
ICC: not tested yet
IHC: not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-NAPG antibody (ABIN1742244)

de Paola, Bello, Michaut: "Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." in: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, (2015) (PubMed).

Burgalossi, Jung, Meyer, Jockusch, Jahn, Taschenberger, OConnor, Nishiki, Takahashi, Brose, Rhee: "SNARE protein recycling by αSNAP and βSNAP supports synaptic vesicle priming." in: Neuron, Vol. 68, Issue 3, pp. 473-87, (2010) (PubMed).

Target Details for NAPG

Target: NAPG (N-Ethylmaleimide-Sensitive Factor Attachment Protein, gamma (NAPG))

Alternative Name: gamma SNAP