Clathrin antibody (AA 156-173)

Catalog No. ABIN1742245

This Mouse Monoclonal antibody specifically detects Clathrin in IHC, WB and ICC. It exhibits reactivity toward Human, Mouse, Rat and Zebrafish (Danio rerio) and has been mentioned in 11+ publications.

Quick Overview for Clathrin antibody (AA 156-173) (ABIN1742245)

Target: Clathrin

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This Clathrin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)

Clone: 57-4

Product Details anti-Clathrin Antibody

Binding Specificity: AA 156-173

Specificity: Specific for both neuronal light chains

Cross-Reactivity (Details): does not cross-react with the non-neuronal variants.

Purification: purified IgG

Immunogen: Synthetic peptide IADKAFYQQPDADTIGYV (aa 156-173) corresponding to the spliced insert of the neuronal light chain variants.

Isotype: IgG1

Application Details

Application Notes: WB: 1 : 5000 up to 1 : 10000 (AP staining)
IP: not tested yet
ICC: 1 : 1000
IHC-P: 1 : 2000

Comment:

Suitable for immunogold electron microscopy.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-Clathrin antibody (ABIN1742245)

Neef, Jung, Wong, Reuter, Pangrsic, Chakrabarti, Kügler, Lenz, Nouvian, Boumil, Frankel, Wichmann, Moser: "Modes and regulation of endocytic membrane retrieval in mouse auditory hair cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 3, pp. 705-16, (2014) (PubMed).

Fuchs, Brandstätter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).

Biesemann, Grønborg, Luquet, Wichert, Bernard, Bungers, Cooper, Varoqueaux, Li, Byrne, Urlaub, Jahn, Brose, Herzog: "Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting." in: The EMBO journal, Vol. 33, Issue 2, pp. 157-70, (2014) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Hellwig, Hack, Kowalski, Brunne, Jarowyj, Unger, Bock, Junghans, Frotscher: "Role for Reelin in neurotransmitter release." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 7, pp. 2352-60, (2011) (PubMed).

Ferguson, Brasnjo, Hayashi, Wölfel, Collesi, Giovedi, Raimondi, Gong, Ariel, Paradise, Otoole, Flavell, Cremona, Miesenböck, Ryan, De Camilli: "A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis." in: Science (New York, N.Y.), Vol. 316, Issue 5824, pp. 570-4, (2007) (PubMed).

Krauss, Kukhtina, Pechstein, Haucke: "Stimulation of phosphatidylinositol kinase type I-mediated phosphatidylinositol (4,5)-bisphosphate synthesis by AP-2mu-cargo complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 32, pp. 11934-9, (2006) (PubMed).

Johnson, Brown, Pulliam, Anderson, Johnson: "Synaptic pathology, altered gene expression, and degeneration in photoreceptors impacted by drusen." in: Investigative ophthalmology & visual science, Vol. 46, Issue 12, pp. 4788-95, (2005) (PubMed).

von Kriegstein, Schmitz: "The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina." in: Cell and tissue research, Vol. 311, Issue 2, pp. 159-73, (2003) (PubMed).

Blumstein, Faundez, Nakatsu, Saito, Ohno, Kelly: "The neuronal form of adaptor protein-3 is required for synaptic vesicle formation from endosomes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 20, pp. 8034-42, (2001) (PubMed).

Von Kriegstein, Schmitz, Link, Südhof: "Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses." in: The European journal of neuroscience, Vol. 11, Issue 4, pp. 1335-48, (1999) (PubMed).

Target Details for Clathrin

Target: Clathrin