NSF antibody

Catalog No. ABIN1742269

This Mouse Monoclonal antibody specifically detects NSF in WB, ELISA, IP, IHC and ICC. It exhibits reactivity toward Rat and Mouse and has been mentioned in 2+ publications.

Quick Overview for NSF antibody (ABIN1742269)

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Reactivity: Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NSF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: 83-11

Product Details anti-NSF Antibody

Specificity: Specific for NSF.

Purification: purified IgG

Immunogen: Recombinant full length rat NSF.

Isotype: IgG1

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 500
IHC-P: 1 : 1000

Comment:

ELISA: Suitable as capture antibody for sandwich-ELISA with ABIN1742270 as detector antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-NSF antibody (ABIN1742269)

Hoopmann, Punge, Barysch, Westphal, Bückers, Opazo, Bethani, Lauterbach, Hell, Rizzoli: "Endosomal sorting of readily releasable synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19055-60, (2010) (PubMed).

Ohno, Ishihara, Terada, Kikuta, Sofue, Kawai, Serikawa, Sasa: "Preferential increase in the hippocampal synaptic vesicle protein 2A (SV2A) by pentylenetetrazole kindling." in: Biochemical and biophysical research communications, Vol. 390, Issue 3, pp. 415-20, (2009) (PubMed).

Target Details for NSF

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Alternative Name: NSF