Neuroligin 3 antibody (AA 608-722)

Catalog No. ABIN1742288

The Rabbit Polyclonal anti-Neuroligin 3 antibody has been validated for WB and ELISA. It is suitable to detect Neuroligin 3 in samples from Rat and Mouse. There are 2+ publications available.

Quick Overview for Neuroligin 3 antibody (AA 608-722) (ABIN1742288)

Target: Neuroligin 3 (NLGN3)

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neuroligin 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Neuroligin 3 Antibody

Binding Specificity: AA 608-722

Specificity: Specific for neuroligin 3. No signal on neuroligin 3 knockouts. Detects other neuroligins when heavily overexpressed.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant mouse neuroligin 3 (aa 608-722).

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: not tested yet
IHC: not tested yet

Comment:

ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742285 as capture antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-Neuroligin 3 antibody (ABIN1742288)

Runkel, Rohlmann, Reissner, Brand, Missler: "Promoter-like sequences regulating transcriptional activity in neurexin and neuroligin genes." in: Journal of neurochemistry, Vol. 127, Issue 1, pp. 36-47, (2013) (PubMed).

Suckow, Comoletti, Waldrop, Mosedale, Egodage, Taylor, Chessler: "Expression of neurexin, neuroligin, and their cytoplasmic binding partners in the pancreatic beta-cells and the involvement of neuroligin in insulin secretion." in: Endocrinology, Vol. 149, Issue 12, pp. 6006-17, (2008) (PubMed).

Target Details for Neuroligin 3

Target: Neuroligin 3 (NLGN3)

Alternative Name: Neuroligin 3

Pathways: Synaptic Membrane