Synaptojanin 1 antibody (Isoform 2)

Catalog No. ABIN1742317

This Rabbit Polyclonal antibody specifically detects Synaptojanin 1 in WB and IHC. It exhibits reactivity toward Rat, Mouse and Hamster.

Quick Overview for Synaptojanin 1 antibody (Isoform 2) (ABIN1742317)

Target: Synaptojanin 1 (SYNJ1)

Reactivity: Rat, Mouse, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Synaptojanin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Synaptojanin 1 Antibody

Binding Specificity: AA 1140-1155, Isoform 2

Specificity: Recognizes isoforms 1, 2, 3 of synaptojanin 1.

Purification: Affinity purified with the immunogen.

Immunogen: Synthetic peptide CGVGAPPSPGVTRREME (aa 1140-1155 in isoform 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 200 up to 1 : 2000 (AP staining)
IP: not tested yet
ICC: not tested yet
IHC: 1 : 1000 up to 1 : 5000

Comment:

Running SDS-PAGE with non-boiled samples gives stronger signals.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Target Details for Synaptojanin 1

Target: Synaptojanin 1 (SYNJ1)

Alternative Name: Synaptojanin 1

Pathways: Inositol Metabolic Process, Synaptic Vesicle Exocytosis