Homer 1/2/3 (N-Term) antibody

Catalog No. ABIN1742340

This anti- antibody is a Rabbit Polyclonal antibody detecting in ICC and WB. Suitable for Human, Mouse, Rat and Zebrafish (Danio rerio). This Primary Antibody has been cited in 2+ publications.

Quick Overview for Homer 1/2/3 (N-Term) antibody (ABIN1742340)

Target: Homer 1/2/3

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Application: Immunocytochemistry (ICC), Western Blotting (WB)

Product Details

Binding Specificity: N-Term

Specificity: The first 120 aa were used for affinity purification. According to Soloviev et al. (2000), they are homologous in Homer 1 a-d, Homer 2 a-d, and Homer 3 a-d.

Purification: Affinity purified with the immunogen.

Immunogen: Purified recombinant N-terminal parts of Homer 1, 2 and 3.

Application Details

Application Notes: WB: 1:1000, ICC: 1:200 up to 1:500, IHC: 1:500,

Comment:

ICC: PFA fixation recommended. Methanol fixation leads to a complete loss of the signal.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50µl H2O to get a 1mg/ml solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C

Storage Comment: Reconstitute and aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized.

References

Tao-Cheng, Thein, Yang, Reese, Gallant: "Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation." in: Neuroscience, Vol. 266, pp. 80-90, (2014) (PubMed).

Tronson, Guzman, Guedea, Huh, Gao, Schwarz, Radulovic: "Metabotropic glutamate receptor 5/Homer interactions underlie stress effects on fear." in: Biological psychiatry, Vol. 68, Issue 11, pp. 1007-15, (2010) (PubMed).

Target Details

Target: Homer 1/2/3

Background: Synonyms: VesL 1, Syn47