RAB27A antibody

Catalog No. ABIN1742354

The Rabbit Polyclonal anti-RAB27A antibody has been validated for WB and IP. It is suitable to detect RAB27A in samples from Mouse and Rat. There are 2+ publications available.

Quick Overview for RAB27A antibody (ABIN1742354)

Target: RAB27A (RAB27A, Member RAS Oncogene Family (RAB27A))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB27A antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-RAB27A Antibody

Specificity: Specific for rab 27A

Cross-Reactivity (Details): no cross reaction to rab 27B.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Purified recombinant full length rat rab 27A.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: not tested yet
IHC: not tested yet

Comment:

Only the non-conserved C-terminal part of the protein was used for affinity purification to achieve specificity.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-RAB27A antibody (ABIN1742354)

Hou, Ernst, Stuenkel, Lentz, Williams: "Rab27A Is Present in Mouse Pancreatic Acinar Cells and Is Required for Digestive Enzyme Secretion." in: PLoS ONE, Vol. 10, Issue 5, pp. e0125596, (2015) (PubMed).

Lovis, Roggli, Laybutt, Gattesco, Yang, Widmann, Abderrahmani, Regazzi: "Alterations in microRNA expression contribute to fatty acid-induced pancreatic beta-cell dysfunction." in: Diabetes, Vol. 57, Issue 10, pp. 2728-36, (2008) (PubMed).

Target Details for RAB27A

Target: RAB27A (RAB27A, Member RAS Oncogene Family (RAB27A))

Alternative Name: Rab 27 A

Pathways: Peptide Hormone Metabolism