GFAP antibody

Catalog No. ABIN1742363

The Rabbit Polyclonal anti-GFAP antibody has been validated for WB, IHC, ELISA, ICC and IP. It is suitable to detect GFAP in samples from Human, Rat, Mouse, Chicken and Zebrafish (Danio rerio). There are 13+ publications available.

Quick Overview for GFAP antibody (ABIN1742363)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Rat, Mouse, Chicken, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-GFAP Antibody

Specificity: Specific for GFAP.

Purification: antiserum

Immunogen: Recombinant full length human GFAP.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 1000
IHC-P: 1 : 1000

Comment:

WB: The polyclonal antibodies are more sensitive compared to the monoclonals. ELISA: Suitable as detector antibody for sandwich-ELISA as capture antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-GFAP antibody (ABIN1742363)

Hüttenrauch, Walter, Kaufmann, Weggen, Wirths: "Limited Effects of Prolonged Environmental Enrichment on the Pathology of 5XFAD Mice." in: Molecular neurobiology, (2016) (PubMed).

Hüttenrauch, Baches, Gerth, Bayer, Weggen, Wirths: "Neprilysin deficiency alters the neuropathological and behavioral phenotype in the 5XFAD mouse model of Alzheimer's disease." in: Journal of Alzheimer's disease : JAD, Vol. 44, Issue 4, pp. 1291-302, (2015) (PubMed).

Esposito, Drexler, Braganza, Doberentz, Grote, Widman, Drosten, Eis-Hübinger, Schoch, Elger, Becker, Niehusmann: "Large-scale analysis of viral nucleic acid spectrum in temporal lobe epilepsy biopsies." in: Epilepsia, Vol. 56, Issue 2, pp. 234-43, (2015) (PubMed).

Cavanagh, Colby-Milley, Bouvier, Farso, Chabot, Quirion, Krantic: "βCTF-correlated burst of hippocampal TNFα occurs at a very early, pre-plaque stage in the TgCRND8 mouse model of Alzheimer's disease." in: Journal of Alzheimer's disease : JAD, Vol. 36, Issue 2, pp. 233-8, (2013) (PubMed).

Kaeser-Woo, Younts, Yang, Zhou, Wu, Castillo, Südhof: "Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 23, pp. 9769-80, (2013) (PubMed).

Hillmann, Hahn, Schilling, Hoffmann, Demuth, Bulic, Schneider-Axmann, Bayer, Weggen, Wirths: "No improvement after chronic ibuprofen treatment in the 5XFAD mouse model of Alzheimer's disease." in: Neurobiology of aging, Vol. 33, Issue 4, pp. 833.e39-50, (2012) (PubMed).

Ansorg, Witte, Urbach: "Age-dependent kinetics of dentate gyrus neurogenesis in the absence of cyclin D2." in: BMC neuroscience, Vol. 13, pp. 46, (2012) (PubMed).

Araya-Callís, Hiemke, Abumaria, Flugge: "Chronic psychosocial stress and citalopram modulate the expression of the glial proteins GFAP and NDRG2 in the hippocampus." in: Psychopharmacology, Vol. 224, Issue 1, pp. 209-22, (2012) (PubMed).

Kawano, Katsurabayashi, Kakazu, Yamashita, Kubo, Kubo, Okuda, Takasaki, Kubota, Mishima, Fujiwara, Harata, Iwasaki: "Long-term culture of astrocytes attenuates the readily releasable pool of synaptic vesicles." in: PLoS ONE, Vol. 7, Issue 10, pp. e48034, (2012) (PubMed).

Ribic, Flügge, Schlumbohm, Mätz-Rensing, Walter, Fuchs: "Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey." in: Behavioral and brain functions : BBF, Vol. 7, pp. 1, (2011) (PubMed).

Christensen, Schneider-Axmann, Lucassen, Bayer, Wirths: "Accumulation of intraneuronal Abeta correlates with ApoE4 genotype." in: Acta neuropathologica, Vol. 119, Issue 5, pp. 555-66, (2010) (PubMed).

Ribic, Zhang, Schlumbohm, Mätz-Rensing, Uchanska-Ziegler, Flügge, Zhang, Walter, Fuchs: "Neuronal MHC class I molecules are involved in excitatory synaptic transmission at the hippocampal mossy fiber synapses of marmoset monkeys." in: Cellular and molecular neurobiology, Vol. 30, Issue 6, pp. 827-39, (2010) (PubMed).

Wirths, Erck, Martens, Harmeier, Geumann, Jawhar, Kumar, Multhaup, Walter, Ingelsson, Degerman-Gunnarsson, Kalimo, Huitinga, Lannfelt, Bayer: "Identification of low molecular weight pyroglutamate A{beta} oligomers in Alzheimer disease: a novel tool for therapy and diagnosis." in: The Journal of biological chemistry, Vol. 285, Issue 53, pp. 41517-24, (2010) (PubMed).

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP