ChT antibody (AA 501-580)

Catalog No. ABIN1742430

This anti-ChT antibody is a Mouse Monoclonal antibody detecting ChT in WB, IHC, IP, ICC. Suitable for Mouse, Human, Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for ChT antibody (AA 501-580) (ABIN1742430)

Target: ChT (High Affinity Choline Transporter (ChT))

Reactivity: Mouse, Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ChT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 62-2E8

Product Details anti-ChT Antibody

Binding Specificity: AA 501-580

Specificity: Specific for the high affinity choline transporter.

Purification: purified IgG

Immunogen: Recombinant protein of rat ChT (aa 501-580).

Isotype: IgG1

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IHC: 1 : 100

Comment:

ChT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-ChT antibody (ABIN1742430)

Misawa, Fujigaya, Nishimura, Moriwaki, Okuda, Kawashima, Nakata, Ruggiero, Blakely, Nakatsu, Ohno: "Aberrant trafficking of the high-affinity choline transporter in AP-3-deficient mice." in: The European journal of neuroscience, Vol. 27, Issue 12, pp. 3109-17, (2008) (PubMed).

Target Details for ChT

Target: ChT (High Affinity Choline Transporter (ChT))

Alternative Name: ChT

Background: Synonyms: Choline transporter, SLC5A7