Abeta 1-40 antibody (AA 33-40)

Catalog No. ABIN1742443

This Rabbit Polyclonal antibody specifically detects Abeta 1-40 in IHC, WB and ELISA. It exhibits reactivity toward Rat, Human and Mouseand has been mentioned in 1 publication.

Quick Overview for Abeta 1-40 antibody (AA 33-40) (ABIN1742443)

Target: Abeta 1-40 (Amyloid beta 1-40 (Abeta 1-40))

Reactivity: Rat, Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Abeta 1-40 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Product Details anti-Abeta 1-40 Antibody

Binding Specificity: AA 33-40

Specificity: Specific for Abeta 40

Cross-Reactivity (Details): no cross reactivity to Abeta 38 and 42.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide corresponding to aa 33-40 of Abeta 40 coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000
IP: not tested yet
ICC: not tested yet
IHC: 1 : 200 up to 1 : 500
IHC-P: 1 : 100 up to 1 : 500

Comment:

WB: Detects purified Abeta 40. Complex samples like brain extracts still have to be tested. ELISA: suitable as capture and detector antibody for sandwich-ELISA (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-Abeta 1-40 antibody (ABIN1742443)

Christensen, Huettenrauch, Mitkovski, Pradier, Wirths: "Axonal degeneration in an Alzheimer mouse model is PS1 gene dose dependent and linked to intraneuronal A? accumulation." in: Frontiers in aging neuroscience, Vol. 6, pp. 139, (2014) (PubMed).

Target Details for Abeta 1-40

Target: Abeta 1-40 (Amyloid beta 1-40 (Abeta 1-40))

Alternative Name: Abeta 40

Background: Synonyms: Beta-App, ABPP