SYCN antibody

Catalog No. ABIN1742468

The Mouse Monoclonal anti-SYCN antibody has been validated for WB. It is suitable to detect SYCN in samples from Mouse and Rat. There are 4+ publications available.

Quick Overview for SYCN antibody (ABIN1742468)

Target: SYCN (Syncollin (SYCN))

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SYCN antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 87-1

Product Details anti-SYCN Antibody

Specificity: Specific for syncollin.

Purification: purified IgG

Immunogen: Recombinant full length syncollin.

Isotype: IgG1

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 5000 (AP staining)
IP: not tested yet
ICC: not tested yet
IHC: not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-SYCN antibody (ABIN1742468)

Bach, Borta, Ackermann, Faust, Borchers, Schrader: "The secretory granule protein syncollin localizes to HL-60 cells and neutrophils." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 54, Issue 8, pp. 877-88, (2006) (PubMed).

Antonin, Wagner, Riedel, Brose, Jahn: "Loss of the zymogen granule protein syncollin affects pancreatic protein synthesis and transport but not secretion." in: Molecular and cellular biology, Vol. 22, Issue 5, pp. 1545-54, (2002) (PubMed).

Kalus, Hodel, Koch, Kleene, Edwardson, Schrader: "Interaction of syncollin with GP-2, the major membrane protein of pancreatic zymogen granules, and association with lipid microdomains." in: The Biochemical journal, Vol. 362, Issue Pt 2, pp. 433-42, (2002) (PubMed).

An, Hansen, Hodel, Jahn, Edwardson: "Analysis of the association of syncollin with the membrane of the pancreatic zymogen granule." in: The Journal of biological chemistry, Vol. 275, Issue 15, pp. 11306-11, (2000) (PubMed).

Target Details for SYCN

Target: SYCN (Syncollin (SYCN))

Alternative Name: Syncollin