TEV (Cut Protease Site) antibody
Reactivity: Tobacco Etch Virus (TEV)
ICC, WB
Host: Rabbit
Polyclonal
unconjugated
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- Target
- TEV
- Binding Specificity
- Cut Protease Site
- Reactivity
- Tobacco Etch Virus (TEV)
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Host
- Rabbit
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Clonality
- Polyclonal
-
Conjugate
- Un-conjugated
- Application
- Immunocytochemistry (ICC), Western Blotting (WB)
- Specificity
- TEV-cut protease site is recognized with very strong preference.
- Purification
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- Immunogen
- ENLYFQ-COOH coupled to KLH via an N-terminal added cysteine.
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- Application Notes
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WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: not tested yet - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Handling Advice
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Storage
- -20 °C
- Storage Comment
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
-
- Target
- TEV
- Target Type
- Virus
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