Glu-alpha-Tubulin (C-Term) antibody

Catalog No. ABIN1742545

This Mouse Monoclonal antibody specifically detects in ELISA, IHC, WB and ICC. It exhibits reactivity toward Human, Mouse, Rat, Eukaryotes and Zebrafish (Danio rerio) and has been mentioned in 9+ publications.

Quick Overview for Glu-alpha-Tubulin (C-Term) antibody (ABIN1742545)

Target: Glu-alpha-Tubulin

Reactivity: Human, Mouse, Rat, Eukaryotes, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)

Clone: 1D5

Product Details

Binding Specificity: C-Term

Specificity: Specific for detyrosinated alpha-tubulin (glu-tubulin) and polyglutamylated tubulin (also beta-tubulin).

Cross-Reactivity (Details): No cross reaction to tyrosinated tubulin.

Immunogen: Synthetic peptide VDSVEGEGEEEGEE (14 C-terminal aa of detyrosinated alpha-tubulin) coupled to BSA via glutaraldehyde. Epitope X-EE including the terminal alpha-carboxylate group. All linkages in the peptide involve alpha-peptide bonds.

Isotype: IgG1

Application Details

Application Notes: WB: 1:1000, ICC: 1:200 up to 1:500, IHC: 1:500, IHC-P: 1:1000,

Comment:

WB: Tween 20 (other detergents not yet tested) should not be added to the blocking and washing solution. It greatly diminishes the intensity of the signal.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100µl H2O to get a 1mg/ml solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C

Storage Comment: Reconstitute and aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized.

References

Zink, Grosse, Freikamp, Bänfer, Müksch, Jacob: "Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells." in: Journal of cell science, Vol. 125, Issue Pt 24, pp. 5998-6008, (2013) (PubMed).

Jablonka, Dombert, Asan, Sendtner: "Mechanisms for axon maintenance and plasticity in motoneurons: alterations in motoneuron disease." in: Journal of anatomy, Vol. 224, Issue 1, pp. 3-14, (2013) (PubMed).

Roll-Mecak, Vale: "Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin." in: Nature, Vol. 451, Issue 7176, pp. 363-7, (2008) (PubMed).

Zhang, Gilquin, Khochbin, Matthias: "Two catalytic domains are required for protein deacetylation." in: The Journal of biological chemistry, Vol. 281, Issue 5, pp. 2401-4, (2006) (PubMed).

Rosales-Nieves, Johndrow, Keller, Magie, Pinto-Santini, Parkhurst: "Coordination of microtubule and microfilament dynamics by Drosophila Rho1, Spire and Cappuccino." in: Nature cell biology, Vol. 8, Issue 4, pp. 367-76, (2006) (PubMed).

Yoon, Shin, Mercurio: "Hypoxia stimulates carcinoma invasion by stabilizing microtubules and promoting the Rab11 trafficking of the alpha6beta4 integrin." in: Cancer research, Vol. 65, Issue 7, pp. 2761-9, (2005) (PubMed).

Van der Heiden, Groenendijk, Hierck, Hogers, Koerten, Mommaas, Gittenberger-de Groot, Poelmann: "Monocilia on chicken embryonic endocardium in low shear stress areas." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 235, Issue 1, pp. 19-28, (2005) (PubMed).

Rüdiger, Rüdiger, Wehland, Weber: "Monoclonal antibody ID5: epitope characterization and minimal requirements for the recognition of polyglutamylated alpha- and beta-tubulin." in: European journal of cell biology, Vol. 78, Issue 1, pp. 15-20, (1999) (PubMed).

Wheatley, Feilen, Yin, Wheatley: "Primary cilia in cultured mammalian cells: detection with an antibody against detyrosinated alpha-tubulin (ID5) and by electron microscopy." in: Journal of submicroscopic cytology and pathology, Vol. 26, Issue 1, pp. 91-102, (1994) (PubMed).

Target Details

Target: Glu-alpha-Tubulin