TUBB3 antibody (AA 443-450)

Catalog No. ABIN1742554

This Guinea Pig Polyclonal antibody specifically detects TUBB3 in WB, IHC, IP and ICC. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 3+ publications.

Quick Overview for TUBB3 antibody (AA 443-450) (ABIN1742554)

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Reactivity: Human, Mouse, Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This TUBB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-TUBB3 Antibody

Binding Specificity: AA 443-450

Specificity: Specific for beta3-tubulin.

Immunogen: Synthetic peptide (aa 443-450 of mouse beta3-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.

Application Details

Application Notes: WB: 1:1000 up to 1:10000, ICC: 1:500, IHC: 1:200, IHC-P: 1:200 up to 1:500,

Comment:

IHC: For best results we recommend antigen retrieval with pepsin according to Lorincz A & Nusser Z (2008).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100µl H2O.

Preservative: Azide and BSA free

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: 4 °C/-20 °C

Storage Comment: Reconstitute and aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized.

References for anti-TUBB3 antibody (ABIN1742554)

Matthäus, Haddjeri, Sánchez, Martí, Bahri, Rovera, Schloss, Lau: "The allosteric citalopram binding site differentially interferes with neuronal firing rate and SERT trafficking in serotonergic neurons." in: European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology, Vol. 26, Issue 11, pp. 1806-1817, (2016) (PubMed).

Andres, Keyser, Petrali, Benton, Hubbard, McNutt, Ray: "Morphological and functional differentiation in BE(2)-M17 human neuroblastoma cells by treatment with Trans-retinoic acid." in: BMC neuroscience, Vol. 14, pp. 49, (2013) (PubMed).

Lau, Heimann, Bartsch, Schloss, Weber: "Nongenomic, glucocorticoid receptor-mediated regulation of serotonin transporter cell surface expression in embryonic stem cell derived serotonergic neurons." in: Neuroscience letters, Vol. 554, pp. 115-20, (2013) (PubMed).

Target Details for TUBB3

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Alternative Name: beta3-Tubulin (TUBB3)

Background: Synonyms: TuJ1

Pathways: Microtubule Dynamics, M Phase