tau antibody (AA 3-214)

Catalog No. ABIN1742567

The Mouse Monoclonal anti-tau antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect tau in samples from Human, Mouse and Rat. There are 2+ publications available.

Quick Overview for tau antibody (AA 3-214) (ABIN1742567)

Target: tau (tau Protein)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This tau antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: 248E5

Product Details anti-tau Antibody

Binding Specificity: AA 3-214

Specificity: Specific for tau.

Purification: purified IgG. Azide was added before lyophilization.

Immunogen: Recombinant mouse tau (aa 3-214).

Isotype: IgG2a

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 200 up to 1 : 500
IHC: 1 : 100

Comment:

WB: Detects the mouse protein with much greater sensitivity than the rat protein. The antibody binds phosphorylated and non-phosphorylated tau proteins.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-tau antibody (ABIN1742567)

Gut, Beske, Hubbard, Lyman, Hamilton, McNutt: "Novel application of stem cell-derived neurons to evaluate the time- and dose-dependent progression of excitotoxic injury." in: PLoS ONE, Vol. 8, Issue 5, pp. e64423, (2013) (PubMed).

Ahmad, Wolber, Eckardt, Koch, Schmitt, Semechkin, Geis, Heckmann, Brüstle, McLaughlin, Sirén, Müller: "Functional neuronal cells generated by human parthenogenetic stem cells." in: PLoS ONE, Vol. 7, Issue 8, pp. e42800, (2012) (PubMed).

Target Details for tau

Target: tau (tau Protein)

Alternative Name: tau