NCK1 antibody

Catalog No. ABIN1774727

The Mouse Monoclonal anti-NCK1 antibody has been validated for WB and ICC. It is suitable to detect NCK1 in samples from Human and Mouse. There is 1 publication available.

Quick Overview for NCK1 antibody (ABIN1774727)

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NCK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Clone: EM-06

Product Details anti-NCK1 Antibody

Purpose: Anti-NCK1 Purified

Specificity: The mouse monoclonal antibody EM-06 recognizes NCK1 (NCK alpha), an ubiquitously expressed cytoplasmic SH2/SH3 adaptor protein important for organization of actin cytoskeleton structures.

Cross-Reactivity (Details): Human, Mouse

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Purified recombinant second SH3 domain of human Nck1 fused to GST

Isotype: IgG1

Application Details

Application Notes: Western blotting: Recommended dilution: 2 μg/mL, positive control: Jurkat cell lysate.
Immunocytochemistry: Recommended dilution: 2 μg/mL, positive control: human NCK1-transfected COS-7 cells.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Do not use after expiration date stamped on vial label.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-NCK1 antibody (ABIN1774727)

Borroto, Arellano, Dopfer, Prouza, Suchànek, Fuentes, Orfao, Schamel, Alarcón: "Nck recruitment to the TCR required for ZAP70 activation during thymic development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 190, Issue 3, pp. 1103-12, (2013) (PubMed).

Target Details for NCK1

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Alternative Name: NCK1

Background: NCK adaptor protein 1,NCK1 (NCK alpha) is a cytoplasmic adaptor protein that plays a universal role in coordinating the signaling networks critical for organizing the actin cytoskeleton, cell movement, or axon guidance, connecting transmembrane receptors to multiple intracellular signaling pathways. It contains one SH2 domain, through which NCK1 binds to phosphorylated domains of transmembrane signaling moleculs or certain adaptor proteins, and three SH3 domains for binding proline-rich sequences of other molecules involved in the process of nucleation and polymerization of the actin cytoskeleton.,NCK-1, NCKalpha

Gene ID: 4690

UniProt: P16333

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals