BU-1A / B6.1 antibody (FITC)

Catalog No. ABIN180731

The Mouse Monoclonal anti-BU-1A / B6.1 antibody has been validated for FACS. It is suitable to detect BU-1A / B6.1 in samples from Chicken.

Quick Overview for BU-1A / B6.1 antibody (FITC) (ABIN180731)

Target: BU-1A / B6.1

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This BU-1A / B6.1 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: L22

Product Details anti-BU-1A / B6.1 Antibody (FITC)

Specificity: MCA2170F recognises the 'a' allele of the polymorphic cell surface antigen Bu-1.

Characteristics: Synonyms: B-Cell Marker, BU-1 Allele A

Purification: Affinity chromatography on Protein G

Immunogen: Chicken Bursa cellsGenename: BU-1A

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 1/20 - 1/80, Use 10 μL of the suggested working dilution to label 106 cells in100 μL. Method sheets are available on request.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: BPS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at +4 °C or at -20 °C if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Should this productcontain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for BU-1A / B6.1

Target: BU-1A / B6.1

Background: Bu-1a is a 70kD alloantigen expressed on B cells and a subset of macrophages. Reports suggest that Bu-1 is expressed on B cell precursors at early stages of development. Weak cross-reactivity with Bu-1b may be seen at higher antibody levels. This may be removed by appropriate titration. Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0-Ag14 myeloma cell line.Synonyms: B-Cell Marker, BU-1 Allele A

Gene ID: 396098

UniProt: Q90746