PRNP antibody

Catalog No. ABIN180947

Product Details anti-PRNP Antibody

Target: PRNP (Prion Protein (PRNP))

Reactivity: Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRNP antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody recognises the scrapie-associated protease-resistant isoform (PrPsc) of ovine prion protein (PrP). Clone 2G11 specifically recognises the Re151-Re159 sequence and does not recognise the non-pathogenic PrPec form.

Characteristics: Synonyms: Major prion protein, PrP27-30, PrP33-35C, ASCR, PRNP, PRIP

Purification: Affinity chromatography on Protein G

Immunogen: Synthetic peptide 146-Re154Re171-182 of ovine PrP

Clone: 2G11

Isotype: IgG2a

Application Details

Application Notes: ELISA: 1/50 - 1/1000, Tested on peptide. Immunohistochemistry on paraffin sections: suitable for use on tissue fixed in 4 % neutralbuffered formalin. Treatment of tissue sections in 98 % formic acid, for 30 minutes, isrecommended prior to pre-treatment with trypsin at 37C for 5 minutes followed by heatmediated retrieval with 10 mM citrate buffer pH 6.1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

References for anti-PRNP antibody (ABIN180947)

Reil, Bux: "Geno- and phenotyping of human neutrophil antigens." in: Methods in molecular biology (Clifton, N.J.), Vol. 1310, pp. 193-203, (2016) (PubMed).

Lederle, Hartenstein, Meides, Kunzelmann, Werb, Angel, Mueller: "MMP13 as a stromal mediator in controlling persistent angiogenesis in skin carcinoma." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1175-84, (2010) (PubMed).

Rolhion, Barnich, Bringer, Glasser, Ranc, Hébuterne, Hofman, Darfeuille-Michaud: "Abnormally expressed ER stress response chaperone Gp96 in CD favours adherent-invasive Escherichia coli invasion." in: Gut, Vol. 59, Issue 10, pp. 1355-62, (2010) (PubMed).

Andréoletti, Berthon, Levavasseur, Marc, Lantier, Monks, Elsen, Schelcher: "Phenotyping of protein-prion (PrPsc)-accumulating cells in lymphoid and neural tissues of naturally scrapie-affected sheep by double-labeling immunohistochemistry." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 50, Issue 10, pp. 1357-70, (2002) (PubMed).

Target Details for PRNP

Target: PRNP (Prion Protein (PRNP))

Alternative Name: CD230 / PrP (PRNP Products)

Background: The prion protein is encoded by the PrP gene and is constitutively expressed as the normal cellular isoform PrPec by many cell types. Transmissible spongiform encephalopathies (TSE) are neurodegenerative diseases in which the accumulation of the abnormal PrPesc isoform in tissues, of the lymphoid and central nervous system, is thought to be a key event. Studies suggest that PrPesc is the causative agent of transmissible spongiform encephalitis (TSE) but this has not been confirmed.Synonyms: ASCR, Major prion protein, PRIP, PRNP, PrP27-30, PrP33-35C

Gene ID: 493887

UniProt: P23907

Pathways: Transition Metal Ion Homeostasis, Activated T Cell Proliferation