Complement Factor I antibody

Catalog No. ABIN181115

The Mouse Monoclonal anti-Complement Factor I antibody has been validated for WB, EIA, IHC (fro) and Func. It is suitable to detect Complement Factor I in samples from Human.

Quick Overview for Complement Factor I antibody (ABIN181115)

Target: Complement Factor I (CFI)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Complement Factor I antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: 3R-8

Product Details anti-Complement Factor I Antibody

Specificity: This antibody recognises complement factor I, a member of the peptidase S1 family present in the blood plasma.

Characteristics: Synonyms: CFI, IF, KAF, AHUS3, C3BINA, C3b-INA, C3B/C4B inactivator

Purification: Affinity chromatography on Protein A

Immunogen: Native human factor I from serum.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/3000 - 1/4000. Western Blot. Functional Assays. Immunohistochemistry on frozen sections. Recommended Positive Control: Kidney from post streptoccal glomerulonephritis patients.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0mg/mL

Buffer: Borate buffered saline pH 8.4 containing 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Complement Factor I

Target: Complement Factor I (CFI)

Alternative Name: Complement Factor I

Background: Factor I is an 88 kDa protein consisting of one polypeptide chain of 35.4 kDa and one of 27.6 kDa. Factor I is a serine protease which inactivates complement components C3b and C4b. C3b cleavage requires the presence of cofactors complement component (3b/4b) receptor 1 (CR1), membrane cofactor protein or factor H, while C4b cleavage requires complement component 4 binding protein or CR1. Factor I has no natural inhibitors. Dysfunctional factor I can lead to uncontrolled activation of the alternative complement pathway causing in systemic depletion of C3 and lower levels of factors B and H, resulting in recurrent pyogenic infections. Defects in CFI are also associated with haemolytic uraemic syndrome.Synonyms: AHUS3, C3B/C4B inactivator, C3BINA, C3b-INA, CFI, IF, KAF

Gene ID: 3426

UniProt: P05156

Pathways: Complement System