IL12A antibody

Catalog No. ABIN181145

Product Details anti-IL12A Antibody

Target: IL12A (Interleukin 12 alpha (IL12A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL12A antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunofluorescence (IF)

Specificity: This antibody detects Human Interleukin-12. Other species not tested.

Characteristics: Synonyms: IL-12A, IL-12 subunit p35, NKSF1, CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDasubunit, NK cell stimulatory factor chain 1

Purification: From Ascites by Affinity Chromatography on Protein A

Immunogen: Highly pure (>98%) recombinant Human IL-12

Isotype: IgG1

Application Details

Application Notes: ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of thisantibody will detect at least 1000 pg/mL of recombinant Human IL-12 when used with Biotinanti-Human IL-12 antibody (cat. PP1102B) as the detection antibody at a concentration ofapproximately 4.0-8.0 μg/mL. Western Blot: To detect Human IL-12 by Western Blot analysis this antibody can be used ata concentration of 0.50-1.0 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hIL-12 is 0.5-1.0 ng/lane, under non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2-8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for IL12A

Target: IL12A (Interleukin 12 alpha (IL12A))

Alternative Name: Interleukin-12 alpha / IL12A (IL12A Products)

Background: IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-γ production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Among its biological activities IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-Gamma producing Th1 cells.Synonyms: CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, NK cell stimulatory factor chain 1, NKSF1

Gene ID: 3592

UniProt: P29459

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation