CCL13 antibody

Catalog No. ABIN181568

This Goat Polyclonal antibody specifically detects CCL13 in WB, EIA and Func. It exhibits reactivity toward Human.

Quick Overview for CCL13 antibody (ABIN181568)

Target: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CCL13 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CCL13 Antibody

Specificity: This antibody detects Macrophage Chemotactic Protein-4.

Purification: Immunoaffinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human MCP-4

Application Details

Application Notes: ELISA: Indirect: To detect hMCP-4 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-4. Sandwich: To detect hMCP-4 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. This In conjunction with BiotinylatedAnti-Human MCP-4 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-4. Western Blot: To detect hMCP-4 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMCP-4 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMCP-4 (100 ng/mL), a concentration of 8 - 12 μg/mL of this antibody is required.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for CCL13

Target: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Alternative Name: MCP-4 / CCL13

Background: Human macrophage/monocyte chemotactic protein 4 (MCP4) is a member of the b-chemokine family (C-C) of Cytokines. Like Eotaxin, MCP4 is a powerful eosinophil chemo-attractant. Additionally, like MCP3, MCP4 elicits chemo-attraction of monocytes and T lymphocytes.Synonyms: C-C motif chemokine 13, CCL13, CK-beta-10, MCP4, Monocyte chemotactic protein 4, NCC-1, NCC1, SCYA13, Small-inducible cytokine A13

Gene ID: 6357

NCBI Accession: NP_005399

UniProt: Q99616

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, The Global Phosphorylation Landscape of SARS-CoV-2 Infection